欧美大屁股眼子xxxxx视频,玩弄老年妇女过程,农村肥bwbwbwbwbw,3d动漫精品啪啪一区二区免费

當前位置:首頁  >  技術文章  >  LHPP抑制PDAC細胞的活力、遷移和增殖,顯著影響SDC1和S100p的表達

LHPP抑制PDAC細胞的活力、遷移和增殖,顯著影響SDC1和S100p的表達

更新時間:2024-12-28  |  點擊率:144

20231月,中國山東第一醫(yī)科大學附屬醫(yī)院肝膽外科 (Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China)研究團隊在《Technology in Cancer Research & Treatment》上發(fā)表論文:

LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p"

 

LHPP抑制PDAC細胞的活力、遷移和增殖,顯著影響SDC1S100p的表達"

 

Abstract

Introduction: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy with a poor response to chemotherapy and an extremely poor prognosis. Recent studies have revealed that phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) can inhibit the growth of various cancers. Therefore, the current study was conducted to investigate the antitumor effects of LHPP in PDAC and to explore its mechanism using proteomics analysis.

Methods and results: Immunohistochemical analysis of clinical samples demonstrated that LHPP expression levels were lower in tumor tissues compared to adjacent nontumor tissues. Moreover, mu ltivariate COX regression analysis showed that LHPP expression level was an independent prognostic factor for the patients with PDAC. Patients with high LHPP expression had a better prognosis. The lentiviral vectors for normal control (NC), LHPP knockdown (KD), and LHPP overexpression (OE) were infected with BxPC-3 and PANC-1 cell lines. Cell counting kit-8 assay, Transwell assay, and flow cytometry analyses showed that LHPP overexpression significantly inhibited the cell viability, migration, and proliferation of BxPC-3 and PANC-1 cells. Moreover, xenograft tumor model demonstrated that LHPP overexpression inhibited xenograft tumor growth in vivo. Subsequently, proteins with significantly altered expression in BxPC-3 cells after lentivirus infection were detected using proteomics analyses. Interestingly, compared to the NC group, the expression of Syndecan 1 (SDC1) was significantly upregulated in the KD group, while that of S100P was significantly downregulated in the OE group.

 

Conclusion: LHPP might emerge as an important target for delaying the advancement of PDAC, thereby providing a novel therapeutic approach for the treatment of PDAC.


摘要:

胰腺導管腺癌(Pancreatic ductal adencarcinoma, PDAC)是一種高度侵襲性的惡性腫瘤,對化療反應差,預后極差。近年來的研究表明,磷酸賴氨酸磷組氨酸無機焦磷酸鹽磷酸酶(LHPP)可以抑制多種癌癥的生長。因此,本研究通過蛋白質(zhì)組學分析,探討LHPPPDAC中的抗腫瘤作用,并探討其作用機制。

方法和結(jié)果:臨床樣本免疫組化分析顯示,腫瘤組織中LHPP表達水平低于鄰近非腫瘤組織。多因素COX回歸分析顯示LHPP表達水平是影響PDAC患者預后的獨立因素。LHPP高表達患者預后較好。用BxPC-3PANC-1細胞株感染正常對照(NC)LHPP敲低(KD)LHPP過表達(OE)慢病毒載體。細胞計數(shù)試劑盒-8、Transwell實驗和流式細胞術分析顯示,LHPP過表達顯著抑制BxPC-3PANC-1細胞的活力、遷移和增殖。此外,異種移植瘤模型表明,LHPP過表達抑制異種移植瘤的體內(nèi)生長。隨后,利用蛋白質(zhì)組學分析檢測慢病毒感染后BxPC-3細胞中表達顯著改變的蛋白。有趣的是,與NC組相比,KDSyndecan 1 (SDC1)的表達顯著上調(diào),OES100P的表達顯著下調(diào)。

結(jié)論:LHPP可能成為延緩PDAC進展的重要靶點,為PDAC的治療提供了一種新的治療途徑。

 

該論文中,人胰腺導管腺癌細胞系(BxPC-3PANC-1)體外培養(yǎng)是使用Ausbian特級胎牛血清完成的。


色欲av人妻精品麻豆av| 黄色视频app下载| 破外女13一14在线观看| 亲嘴视频| 欧美特黄a级高清免费大片a片| 婷婷丁香五月久久综合啪啪图区 | 供人泄欲玩弄的妓女h| 爽灬爽灬爽灬毛及a片| 肥嫩多毛的大荫户| 亚洲熟女乱色综合亚洲图片| 翁与小莹浴室欢爱52章| 人人妻人人澡人人爽人人dvd | 一区二区亚洲av天堂嫩模| 女教师の爆乳bd在线观看| 暴力调教一区二区三区| 国产边打电话边做对白在线| 人妻少妇屁股翘水多| 当着全班面被c到高潮哭视频| 日本在线观看| 国自产拍偷拍精品啪啪一区二区| 亚洲国产日韩a在线欧美2020| 黑人bbcvideos极品| 受被攻c哭高h视频网站| 国偷自产av一区二区三区123| 欧美激情视频| 校草喝下春药被男生玩弄| 婷婷丁香五月| 名门嫡姝-213大h慎入| 久久一区二区三区精华液| 免费高清视频| 人人澡超碰碰97碰碰碰| 精品少妇无码av无码专区| 蜜芽va亚洲va欧美va天堂| 国产男男激情videosgay| 亚洲无线码一区国产欧美国日产 | 囗交姿势图3d效果展示图| 啊灬啊别停灬用力啊村妇| sm抽打调教女人光屁股的作文| 换着玩人妻hd中文字幕| 免费人妻精品一区二区三区| 惨遭蹂躏的大学校花|